Part:BBa_K1970002
Red fluorescent protein controlled by CI-repressor
This part is made up of a gene for red fluorescent protein (mCherry) controlled by the lambda promoter. A CI-repressor gene is attached to the parts ([1]) , which represses expression of mCherry by binding to the lambda promoter.
Usage and Biology
The sensitivity of the CI repressor towards temperature in this biobrick was tested by growing overnight cultures inoculated with E. coli DH5alpha cells at 30 °C and 37 °C. After correcting for cell density in the cultures, the fluorescence of each culture was measured:
Excitation wavelength: 584 nm, emission wavelength: 620 nm
Average fluorescence at 30 °C was 3413
Average fluorescence at 37 °C was 13700
These results show that the CI repressor implemented in this part is effective at repressing gene expression when grown at 30 °C.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 781 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1586
Illegal AgeI site found at 1698 - 1000COMPATIBLE WITH RFC[1000]
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